Amin, Z., Imran, M., Shamim, A., Sajid, M., Rizwan, H., Rehman, T., Shah, S. (2024). Analysis of immunodiagnostic potency of excretory secretory antigens of Haemonchus contortus by SDS-PAGE. Parasitologists United Journal, 17(3), 211-213. doi: 10.21608/puj.2024.313940.1265
Zubaria Amin; Muhammad Imran; Asim Shamim; Muhammad Sajid; Hafiz Rizwan; Tanzeel Rehman; Syed Shah. "Analysis of immunodiagnostic potency of excretory secretory antigens of Haemonchus contortus by SDS-PAGE". Parasitologists United Journal, 17, 3, 2024, 211-213. doi: 10.21608/puj.2024.313940.1265
Amin, Z., Imran, M., Shamim, A., Sajid, M., Rizwan, H., Rehman, T., Shah, S. (2024). 'Analysis of immunodiagnostic potency of excretory secretory antigens of Haemonchus contortus by SDS-PAGE', Parasitologists United Journal, 17(3), pp. 211-213. doi: 10.21608/puj.2024.313940.1265
Amin, Z., Imran, M., Shamim, A., Sajid, M., Rizwan, H., Rehman, T., Shah, S. Analysis of immunodiagnostic potency of excretory secretory antigens of Haemonchus contortus by SDS-PAGE. Parasitologists United Journal, 2024; 17(3): 211-213. doi: 10.21608/puj.2024.313940.1265
Analysis of immunodiagnostic potency of excretory secretory antigens of Haemonchus contortus by SDS-PAGE
1Departments of Livestock and Dairy Development, Animal Science Institute, Manager Production, Hi Tech Feed and Oil Mills, Quetta, Balochistan
2Departments of Parasitology, Faculty of Veterinary Science, University of Agriculture ,Manager Production, Hi Tech Feed and Oil Mills, Quetta, Faisalabad
3Faculty of Veterinary and Animal Sciences , Manager Production, Hi Tech Feed and Oil Mills, Azad Kashmir3, Pakistan
4University of Agriculture , Manager Production, Hi Tech Feed and Oil Mills, Quetta Faisalabad ,Pakistan
5Manager Production, Hi Tech Feed and Oil Mills, Quetta , Lahore ,Pakistan
6Manager Production, Hi Tech Feed and Oil Mills, Quetta ,Sahiwal , Pakistan
7Faculty of Veterinary and Animal Sciences, University of Poonch , Pathobiology (Parasitology)section, Manager Production, Hi Tech Feed and Oil Mills, Quetta ,Rawalakot, Azad Kashmir , Pakistan
Abstract
Background: Helminthic excretory secretory (E/S) antigens gained considerable interest due to their role in stimulating host-parasite interactions and as potential candidate for vaccine development. Objective: To quantify and characterize H. contortus E/S antigens aiming for its use as an effective control measure. Material and Methods: Adult worms were collected from the abomasa of slaughtered goats. Fresh and highly motile adult worms were transferred to RPMI 1640 culture medium containing antibiotics. The E/S antigen was procured through standard protocols and concentration of proteins was measured. Characterization of ES antigen was subjected to SDS-PAGE and estimation of protein concentration. Results: The triturate contained proteins with a concentration of 0.836 mg/ml. Three bands of proteins appeared on the gel with molecular weights of 65, 48 and 30 kDa. Conclusion: Further characterization of E/S antigens will be helpful in developing effective control measures against the worms in general and haemonchosis specifically.
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