Determination of the post-mortem interval using forensically important Sarcophagidae

Document Type : Original Article

Authors

1 Departments of Medical Parasitology Faculties of Medicine, Benha Universities, Qaliobyia, Egypt

2 Departments of Forensic Medicine and Clinical Toxicology , Suez Canal Universities, Qaliobyia, and Ismailia, Egypt

Abstract

Background: Estimating the age of Sarcophagidae’ pupa is a challenge in forensic entomology because
the postmortem anatomical and morphological changes are not easily determined. Therefore, accurate
estimation of pupal age is crucial for assessing the minimum postmortem interval (minPMI).
Objective: To detect the insect concerned with decomposition of dead animal remains in the Egyptian
environment, specifically focusing on the life stages at the time of collection and the time it takes for the
colonizing insects to complete their development.
Material and Methods: Sarcophaga life cycle stages collected from distributed animal tissues and buried
animal carcasses were identified. Their characteristics were recorded microscopically, and the duration
needed for the initial colonizing insects to develop and reach their full size or life stage at the time of
collection was determined.
Results: Collected Sarcophagidae larvae, pupae, and adults were identified. Estimation of the periods
of their emergence and climate conditions showed that three larval instars appeared from day 2 till day
8, then pupae appeared on day 8 and finally adults emerged at day 16. The pupal period constituted
approximately 50% of immature development.
Conclusion: Determining the pre-pupal and intra-puparial development of household Sarcophagidae
enables reliable estimation of minPMI which is valuable in forensic entomology.

Keywords