Abd Ellah, A., GabAllah, M. (2024). Genotyping of Sarcocystis spp. in bovine carcasses from slaughterhouses in Sohag, Egypt. Parasitologists United Journal, 17(2), 121-125. doi: 10.21608/puj.2024.297600.1252
Asmaa Abd Ellah; Manal GabAllah. "Genotyping of Sarcocystis spp. in bovine carcasses from slaughterhouses in Sohag, Egypt". Parasitologists United Journal, 17, 2, 2024, 121-125. doi: 10.21608/puj.2024.297600.1252
Abd Ellah, A., GabAllah, M. (2024). 'Genotyping of Sarcocystis spp. in bovine carcasses from slaughterhouses in Sohag, Egypt', Parasitologists United Journal, 17(2), pp. 121-125. doi: 10.21608/puj.2024.297600.1252
Abd Ellah, A., GabAllah, M. Genotyping of Sarcocystis spp. in bovine carcasses from slaughterhouses in Sohag, Egypt. Parasitologists United Journal, 2024; 17(2): 121-125. doi: 10.21608/puj.2024.297600.1252
Genotyping of Sarcocystis spp. in bovine carcasses from slaughterhouses in Sohag, Egypt
Department of Medical Parasitology, Faculty of Medicine, Sohag University, Sohag, Egypt
Abstract
Background: The prevalence of bovine sarcocystosis is high in most regions of the world. In Egypt, it constitutes a major human health problem due to consumption of insufficiently cooked bovine meat. Objective: To determine the detection rate and species identity of Sarcocystis infection in slaughtered water buffaloes (Bubalus bubalis) in Egypt. Material and Methods: Samples from esophagus and tongue were collected from 100 slaughtered buffaloes from different slaughterhouses in Sohag, Egypt and subjected to macroscopical, microscopical, and histopathological examinations. The genomic DNA was extracted from Sarcocystis-infected muscle samples and PCR–RFLP method was used to amplify partial parts of the gene encoding 18S rRNA. The PCR products were digested by restriction endonuclease enzyme BclI for species identification. Results: Macroscopic sarcocysts were detected in 25% of slaughtered buffaloes, however, no microscopic sarcocysts were detected. Molecular analysis resulted in successful amplification of all positive samples and S. fusiformis was identified in all positive samples. Conclusion: All isolates obtained from slaughtered buffaloes in Sohag, Egypt were S. fusiformis suggesting the potential role of cats in the transmission of S. fusiformis. Our study recommends strict hygienic measures to protect animals and humans from infection.
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