El Naggar, H., Shehata, M., Abdelmaksoud, H., Barakat, A., Abdelhameed, R., Mohammad, O., El-Ashkar, A. (2024). Therapeutic potential of coconut and ginger oils in chronic murine toxoplasmosis. Parasitologists United Journal, 17(1), 37-45. doi: 10.21608/puj.2024.273767.1239
Heba El Naggar; Mai Shehata; Hagar Abdelmaksoud; Ashraf Barakat; Reda Abdelhameed; Omnia Mohammad; Ayman El-Ashkar. "Therapeutic potential of coconut and ginger oils in chronic murine toxoplasmosis". Parasitologists United Journal, 17, 1, 2024, 37-45. doi: 10.21608/puj.2024.273767.1239
El Naggar, H., Shehata, M., Abdelmaksoud, H., Barakat, A., Abdelhameed, R., Mohammad, O., El-Ashkar, A. (2024). 'Therapeutic potential of coconut and ginger oils in chronic murine toxoplasmosis', Parasitologists United Journal, 17(1), pp. 37-45. doi: 10.21608/puj.2024.273767.1239
El Naggar, H., Shehata, M., Abdelmaksoud, H., Barakat, A., Abdelhameed, R., Mohammad, O., El-Ashkar, A. Therapeutic potential of coconut and ginger oils in chronic murine toxoplasmosis. Parasitologists United Journal, 2024; 17(1): 37-45. doi: 10.21608/puj.2024.273767.1239
Therapeutic potential of coconut and ginger oils in chronic murine toxoplasmosis
1Departments of Medical Parasitology , Faculty of Medicine, Ain Shams University , Cairo,
2Departments of Medical Parasitology1, Faculty of Medicine, Ain Shams University1, Cairo,
3Theodor Bilharz Research Institute
4Giza; Zoonotic Diseases, National Research Centre
5Applied Organic Chemistry, Chemical Industries Research Institute , Giza; Egypt
6Departments of Medical Parasitology , Faculty of Medicine, Ain Shams University
Abstract
Background: Since current therapies for toxoplasmosis are only effective against tachyzoites in acute infections, and the occurrence of drug resistance, researches are directed to use natural products in the treatment of chronic toxoplasmosis. Objective: To investigate the potential efficacy of metal-organic framework (MOF) loaded coconut oil (CO) and ginger oil (GO) nanoparticles (NPs) in the treatment of chronic murine toxoplasmosis. Material and Methods: Ninety laboratory bred Swiss Albino mice were divided into 8 groups (10 mice each); GI (negative control), GII (infected control), GIII-GVIII (infected with Me49 strain of T. gondii and treated with MOFs-NPs, Spiramycin, Spiramycin loaded on MOFs-NPs, CO-MOF-NPs, GO-MOF-NPs, and CO+GO-MOFs-NPs, respectively). Parameters used for evaluation included brain cyst count, tissue pathology, and CD8+ infiltration of the liver. Results: A statistically significant difference was observed in the number of brain cysts between all infected groups receiving treatment, and GII; and GV showed the lowest count of brain cysts with a 60.8% reduction. Histopathological examination showed that loading CO and GO separately or combined with MOFs-NPs significantly restored the normal architecture of all examined tissues, i.e., brain, eye, liver, and kidney. Using immunohistochemical (IHC) staining, high-density CD8+ infiltration was recorded in the liver section of both GI and GII. While GIII, GIV, and GVI displayed low-density CD8+ infiltration, GV, GVII, and GVIII showed intermediate-density. Conclusion: Loading on MOFs-NPs, CO, and GO offered promising phytotherapy against chronic toxoplasmosis.
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