Habib, F., Abdel-Fattah, N., Saad, G., El Naggar, H. (2018). Growth kinetics and metronidazole sensitivity of Blastocystis spp. isolated from colorectal carcinoma (CRC) and non-CRC patients. Parasitologists United Journal, 11(3), 175-180. doi: 10.21608/puj.2018.6489.1027
Fayza Habib; Nashwa Abdel-Fattah; Ghada Saad; Heba El Naggar. "Growth kinetics and metronidazole sensitivity of Blastocystis spp. isolated from colorectal carcinoma (CRC) and non-CRC patients". Parasitologists United Journal, 11, 3, 2018, 175-180. doi: 10.21608/puj.2018.6489.1027
Habib, F., Abdel-Fattah, N., Saad, G., El Naggar, H. (2018). 'Growth kinetics and metronidazole sensitivity of Blastocystis spp. isolated from colorectal carcinoma (CRC) and non-CRC patients', Parasitologists United Journal, 11(3), pp. 175-180. doi: 10.21608/puj.2018.6489.1027
Habib, F., Abdel-Fattah, N., Saad, G., El Naggar, H. Growth kinetics and metronidazole sensitivity of Blastocystis spp. isolated from colorectal carcinoma (CRC) and non-CRC patients. Parasitologists United Journal, 2018; 11(3): 175-180. doi: 10.21608/puj.2018.6489.1027
Growth kinetics and metronidazole sensitivity of Blastocystis spp. isolated from colorectal carcinoma (CRC) and non-CRC patients
Medical Parasitology Department, Faculty of Medicine, Ain-Shams University, Cairo, Egypt
Abstract
Background: Blastocystis spp. is the most common protist detected in human fecal samples in numerous studies globally.Until recently debate regarding its pathogenicity has been controversial. Some studies speculated a possible correlation between Blastocystis and colorectal carcinoma (CRC). No previous studies investigated the presence of non-genotypic differences in Blastocystis spp. isolated from CRC and non-CRC patients. Objectives:The present work aimed to investigate the growth kinetics (GK) and metronidazole (MTZ) sensitivity of Blastocystis isolates from CRC and apparent non-CRC symptomatic and asymptomatic Blastocystis carriers. Materials and Methods: Seven isolates from CRC patients, 6 from symptomatic and 6 from asymptomatic non-CRC carriers were cultured in Locke's Egg (LE) medium supplemented with bovine serum and antibiotic mixture, and incubated at 37°C. Mean viable organisms counts of each isolate were calculated every 24 h to follow their GK. Counts were repeated after exposure to different MTZ concentrations to test their drug sensitivity. Results: In vitro GK of CRC and non-CRC symptomatic isolates were nearly similar at 72 h incubation with apparently higher peaks attained by isolates from CRC patients than the slower growing non-CRC-asymptomatic isolates. MTZ-sensitivity of CRC isolates was nearly similar to that of non-CRC asymptomatic isolates; both were significantly more sensitive than the symptomatic isolates especially at high drug concentration of 200 µg/ml of the medium. Conclusion: The recorded difference in GK and MTZ sensitivity of Blastocystis spp. isolated from different clinical groups suggests the existence of certain biological characters with different pathogenic roles.
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