Etewa, S., Al-Hoot, A., Sharaf, H., Moawad, H., Mohamed, S., Alshafey, M., Senosy, H., Sarhan, M. (2019). Modelling approaches to predict and evaluate schistosomiasis immunization utilizing SEA loaded on chitosan nanoparticles via liver tissue differentiation and angiogenesis. Parasitologists United Journal, 12(3), 197-208. doi: 10.21608/puj.2019.16532.1051
Samia Etewa; Abd-Allah Al-Hoot; Hesham Sharaf; Howayda Moawad; Samira Mohamed; Mahmoud Alshafey; Huda Senosy; Mohamed Sarhan. "Modelling approaches to predict and evaluate schistosomiasis immunization utilizing SEA loaded on chitosan nanoparticles via liver tissue differentiation and angiogenesis". Parasitologists United Journal, 12, 3, 2019, 197-208. doi: 10.21608/puj.2019.16532.1051
Etewa, S., Al-Hoot, A., Sharaf, H., Moawad, H., Mohamed, S., Alshafey, M., Senosy, H., Sarhan, M. (2019). 'Modelling approaches to predict and evaluate schistosomiasis immunization utilizing SEA loaded on chitosan nanoparticles via liver tissue differentiation and angiogenesis', Parasitologists United Journal, 12(3), pp. 197-208. doi: 10.21608/puj.2019.16532.1051
Etewa, S., Al-Hoot, A., Sharaf, H., Moawad, H., Mohamed, S., Alshafey, M., Senosy, H., Sarhan, M. Modelling approaches to predict and evaluate schistosomiasis immunization utilizing SEA loaded on chitosan nanoparticles via liver tissue differentiation and angiogenesis. Parasitologists United Journal, 2019; 12(3): 197-208. doi: 10.21608/puj.2019.16532.1051
Modelling approaches to predict and evaluate schistosomiasis immunization utilizing SEA loaded on chitosan nanoparticles via liver tissue differentiation and angiogenesis
1Departments of Medical Parasitology Zagazig University, Zagazig 44519, Egypt
2Departments of Zoology and Clinical Faculties of Medicine , Zagazig University, Zagazig 44519, Egypt
3Departments of Medical Parasitology Zagazig University, Zagazig 44519, Egypt
4Departments of Medical Parasitology Zagazig University, Zagazig 44519, Egypt
5Departments Clinical Pathology , Faculties of Medicine , Zagazig University, Zagazig 44519, Egypt,
6Departments of Zoology Science , Zagazig University, Zagazig 44519, Egypt
7Departments of Medical Parasitology Zagazig University, Zagazig 44519, Egypt,
Abstract
Background: Anti-schistosome vaccination is a necessary approach to minimize the hepatic vascular changes that lead to hepatic pathological consequences. Objective: To assess the prophylactic impact of soluble egg antigen (SEA) loaded on chitosan nanoparticles (ChNPs) on hepatic vascular and pathological consequences in experimental schistosomiasis. Material and Methods: Seventy male Swiss albino mice were classified into 7 groups; each of 10. G1: Non-infected control; G2: Infected control group; G3: Injected by ChNPs then infected subcutaneously (SC) with S. mansoni cercaria; G4: Injected by Freund’s Complete Adjuvant (FCA) then infected; G5: Injected by crude schistosomal egg antigen (SEA) combined with FCA (SEA-FCA) then infected; G6: Injected by SEA loaded on ChNPs (SEA-ChNPs) then infected; G7: Injected by both SEA-FCA + SEA-ChNPs then infected. Evaluation was done by parasitological, histopathological and immunohistochemical studies in murine models challenged by Schistosoma mansoni infection. Results: SEA-ChNPs was more successful in reducing stools and liver egg counts, hepatic granulomas number and size, improving hepatic architecture and vasculature, minimizing hepatic fibrosis, enhancing angiogenesis constructive impact, ameliorating adverse effects during fibrogenesis and remodeling of hepatic tissue by fibrosis degradation than SEA-FCA. Conclusion: ChNPs potentiated the protective and immune impact of SEA as proved by parasitological, histopathological and immunohistochemical assays; and confirmed its specific, marked, supportive and constructive effects on hepatic angiogenesis
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