Document Type : Original Article
Authors
1
medical parasitology-faculty of medicine-sohag university-sohag
2
Department of Medical Parasitology, Faculty of Medicine, Sohag University, Sohag, Egypt
3
Department of Urology, Faculty of Medicine, Sohag University, Sohag, Egypt.
4
Department of Urology, Faculty of Medicine, Azhar Assiut University, Assiut, Egypt.
Abstract
Background: Schistosoma miRNAs and their target genes are utilized in diagnosis and/or prognosis of schistosomiasis.
Objective: To evaluate the diagnostic and prognostic role of Sh-miRNA-71a and its target gene MAPK3 in bilharzial bladder cancer (BC) in Egypt.
Subjects and Methods: This case-control study recruited 40 patients with S. haematobium aged 17-70, of both sexes, and 20 apparently healthy volunteers of similar age range, and gender. Based on clinical examination, and pathological characteristics, patients were divided into two equal groups: BC, and chronic bilharzial cystitis. From each patient with BC, a histopathological report for diagnosis and grading was obtained. Urine samples were collected from all participants, and miRNA, and MAPK3 RNA were extracted, followed by cDNA synthesis, amplification, and Sh-miRNA-71a and MAPK3 quantification using real time PCR (RT-PCR) assay. The obtained results were correlated with BC type and grade.
Results: Tested Sh-miRNA-71a was significantly higher in the bilharzial BC group than the chronic cystitis (P=0.005). The MAPK3 was significantly higher in patients with bilharzial BC than those with chronic cystitis, and controls. It was also significantly (P<0.05) higher in chronic cystitis, than the controls. In addition, Sh-miRNA-71a and MAPK3 were significantly (P<0.001) higher in patients with high-grade bilharzial BC than in patients with low-grade bilharzial BC. There was a positive correlation between MAPK3 and Sh-miRNA-71a (r=0.313, P=0.049) indicating that both biomarkers can significantly predict malignancy and differentiate between high- and low-grade malignancy.
Conclusion: Urinary Sh-miRNA-71a and its target gene MAPK3 can be used as non-invasive prognostic markers to predict bilharzial BC and differentiate between high- and low-grade cases with positive significant correlation.
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