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Hegazy, M., Gomaa, A., Saleh, N. (2025). Loop-mediated isothermal amplification for protozoan diagnosis: Review of ongoing development and implementations. Parasitologists United Journal, 18(1), 1-20. doi: 10.21608/puj.2025.338230.1278
Mona K Hegazy; Ahmed M Gomaa; Nora E Saleh. "Loop-mediated isothermal amplification for protozoan diagnosis: Review of ongoing development and implementations". Parasitologists United Journal, 18, 1, 2025, 1-20. doi: 10.21608/puj.2025.338230.1278
Hegazy, M., Gomaa, A., Saleh, N. (2025). 'Loop-mediated isothermal amplification for protozoan diagnosis: Review of ongoing development and implementations', Parasitologists United Journal, 18(1), pp. 1-20. doi: 10.21608/puj.2025.338230.1278
Hegazy, M., Gomaa, A., Saleh, N. Loop-mediated isothermal amplification for protozoan diagnosis: Review of ongoing development and implementations. Parasitologists United Journal, 2025; 18(1): 1-20. doi: 10.21608/puj.2025.338230.1278

Loop-mediated isothermal amplification for protozoan diagnosis: Review of ongoing development and implementations

Article 1, Volume 18, Issue 1, April 2025, Page 1-20  XML PDF (736.23 K)
Document Type: Review Article
DOI: 10.21608/puj.2025.338230.1278
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Authors
Mona K Hegazy email orcid 1; Ahmed M Gomaa2; Nora E Saleh3
1Medical Parasitology Department, at Mansoura faculty of Medicine, Egypt.
2Department of Medical Parasitology, Faculty of Medicine, Mansoura University, Mansoura, 35516, Egypt
3Department of Medical Parasitology, Faculty of Medicine, Mansoura University
Abstract
Parasitic protozoa are emerging as global health risks worldwide. Accurate screening of protozoal infection is pivotal for an effective strategic control process. Traditional parasitological techniques, immunodiagnostics and PCR-based methods are the classical tools for diagnosing protozoal infection, even so they are labor and time-intensive, expensive, and occasionally give inconsistent results. The loop mediated isothermal amplification (LAMP) assay, is a single-step amplification reaction that is an extremely sensitive, efficient and robust technique for identification of protozoa. This review aims to offer an overview of the present status of LAMP technology in protozoan diagnosis and to highlight this innovative tool and its future progress through comparison with other traditional diagnostic techniques. Moreover, LAMP
Keywords
amplification; DNA; isothermal conditions; LAMP; molecular diagnostics; PCR; primers; protozoa
Main Subjects
Diagnostic techniques
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