Shehata, M., El Naggar, H., Abdelmaksoud, H., El-Ashkar, A., Barakat, A., Abdelhameed, R., Atta, S., Mohammad, O. (2024). Immunomodulatory impact of natural oils loaded metalorganic frameworks on chronic toxoplasmosis in mice. Parasitologists United Journal, 17(3), 240-246. doi: 10.21608/puj.2024.331310.1275
Mai Shehata; Heba El Naggar; Hagar Abdelmaksoud; Ayman El-Ashkar; Ashraf Barakat; Reda Abdelhameed; Shimaa Atta; Omnia Mohammad. "Immunomodulatory impact of natural oils loaded metalorganic frameworks on chronic toxoplasmosis in mice". Parasitologists United Journal, 17, 3, 2024, 240-246. doi: 10.21608/puj.2024.331310.1275
Shehata, M., El Naggar, H., Abdelmaksoud, H., El-Ashkar, A., Barakat, A., Abdelhameed, R., Atta, S., Mohammad, O. (2024). 'Immunomodulatory impact of natural oils loaded metalorganic frameworks on chronic toxoplasmosis in mice', Parasitologists United Journal, 17(3), pp. 240-246. doi: 10.21608/puj.2024.331310.1275
Shehata, M., El Naggar, H., Abdelmaksoud, H., El-Ashkar, A., Barakat, A., Abdelhameed, R., Atta, S., Mohammad, O. Immunomodulatory impact of natural oils loaded metalorganic frameworks on chronic toxoplasmosis in mice. Parasitologists United Journal, 2024; 17(3): 240-246. doi: 10.21608/puj.2024.331310.1275
Immunomodulatory impact of natural oils loaded metalorganic frameworks on chronic toxoplasmosis in mice
1Departments of Medical Parasitology, Faculty of Medicine, Ain Shams University, Cairo
2Theodor Bilharz Research Institute, Giza
3Departments of Medical Parasitology, Faculty of Medicine, Ain Shams University, Cairo & Microorganisms and Clinical Parasitology, College of Medicine, University of Bisha, Bisha, Saudi Arabia
4Zoonoti Diseases , National Research Centre, Giza , Egypt
5Applied Organic Chemistry, Chemical Industries Research Institute National Research Centre
6Theodor Bilharz Research Institute, Giza , Egypt
Abstract
Background: Although several drugs and natural products were investigated in the treatment of chronic toxoplasmosis, brain cysts are still resistant to eradication. Objective: To evaluate the immunomodulatory effect of Nigella sativa oil (NSO) and wheat germ oil (WGO) loaded metal-organic frameworks (MOF) on chronic toxoplasmosis in mice. Material and Methods: Eighty male Swiss albino mice were divided into 8 groups (10 mice each); GI: noninfected non treated, GII-GVIII: infected with ME-49 avirulent strain of T. gondii; GII: non-treated, GIII-GVIII were treated with Spiramycin (GIII), Spiramycin-MOF (GIV), MOF (GV), WGO-MOF (GVI), NSO-MOF (GVII), and WGO+NSO-MOF (GVIII). Immunological evaluation was performed through immunohistochemical (IHC) staining of liver sections, and serological detection of immunological markers, i.e., interferon-gamma (INF–γ), and tumor necrosis factor-alpha (TNF–α). Results: The IHC examination revealed a high predominance of CD8+ cells elicited in GVII, and GVIII followed by GVI. A statistically significant difference was recorded between the treated test and control groups regarding the levels of INF–γ, and TNF–α in their sera with the highest level recorded in GVII followed by GVIII, GVI, GIII, GIV, and GV, respectively. Conclusion: Our study showed that NSO and WGO combinations with MOF exhibited significant immunostimulant effects against chronic murine toxoplasmosis.
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