Evaluation of multiplex PCR assay against microscopy in diagnosing Entamoeba histolytica in stool samples at Assiut Governorate Hospitals, Egypt

Document Type : Original Article

Authors

Department of Medical Parasitology, Faculty of Medicine, Assiut University, Assiut, Egypt

Abstract

Background: Cysts of E. histolytica, E. dispar, E. moshkovskii, and others are not microscopically
distinguishable. Since not all are pathogenic to human, unnecessary prescription of anti-amoebic therapy
may lead to drug resistance.
Objective: To evaluate usefulness of the molecular assays to differentiate between pathogenic and nonpathogenic
Entamoeba species.
Patients and Methods: Fecal samples were collected and examined from 187 patients suffering from
diarrhea and/or dysentery, and attending the outpatient clinics of the Assiut Governorate Hospitals. Stool
samples were examined by saline wet mount preparations, iodine, methylene blue and trichrome stains.
Positive stool samples for Entamoeba cysts were preserved for multiplex PCR (mPCR) assay.
Results: Among 187 samples, 24 (12.8%) were positive for Entamoeba complex by microscopic
examination. Multiplex PCR detected E. histolytica, E. moshkovskii and E. dispar in 5 samples (2.7%), 2
samples (1.1%), and one sample (0.5%), respectively.
Conclusion: Multiplex PCR is more suitable for differentiating between Entamoeba spp. for better
treatment strategies and successful control.

Keywords

Parasitologists United Journal
Volume 17, Issue 3
December 2024
Pages 205-210

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