Document Type : Original Article
Authors
1
Departments of Medical Parasitology , Faculty of Medicine , Menoufia University, Shebin El-Kom, Menoufia Governorate, Egypt
2
Departments of Public Health and Community Medicine , Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia Governorate, Egypt
3
Departments of Pathology, National Liver Institute Menoufia University, Shebin El-Kom, Menoufia Governorate, Egypt
4
Departments of Clinical Pathology National Liver Institute ,Menoufia University, Shebin El-Kom, Menoufia Governorate, Egypt
5
Departments of Medical Parasitology, Faculty of Medicine,Menoufia University, Shebin El-Kom, Menoufia Governorate, Egypt
6
Departments of Medical Parasitology,Faculty of Medicine Menoufia University, Shebin El-Kom, Menoufia Governorate, Egypt
Abstract
Background: Curcumin (Cur) exhibits anti-parasitic effects in spite of its poor bioavailability. However,
several studies documented use of nanotechnology as efficient drug delivery system.
Objective: To investigate the effect of Cur nanoparticles (Cur-Nano) alone and in combination with
albendazole (ABZ) on murine trichinosis.
Material and Methods: A total of 150 laboratory-bred, male Swiss Albino mice were divided randomly
and equally into three groups; (I) the intestinal group; (II) the early-treated muscular group; and (III) the
late-treated muscular group. Each group was subdivided equally into five subgroups; (a) negative control
group; (b) infected non-treated (positive control); (c) mice infected and treated by ABZ; (d) mice infected
and treated by Cur-Nano and (e) mice infected and treated by both drugs. Mice were sacrificed on the
7th day post-infection (dpi) (GI) and on the 49th dpi (GII and GIII). The intestinal and muscle specimens
were used for H&E, Masson’s trichrome, and toluidine blue. Additional study parameters included levels
of creatine kinase (CK) to assess degree of muscle destruction, expression levels of vascular endothelial
growth factor (VEGF) as well as serum and muscle matrix metalloproteinase 9 (MMP9) to investigate Cur
anti-angiogenetic activity, and malonaldehyde (MDA), and total antioxidant capacity (TAC) to determine
its anti-oxidant effects.
Results: All treated subgroups showed a significant reduction in adult worm and larval count, with
the highest reduction of 99.325 % and 95.94% in GIe and GIIIe respectively. There was a significant
improvement in the inflammatory response with significantly decreased expression of VEGF. In both early
and late treated mice, serum CK, MDA and MMP9 levels were decreased with improved TAC levels in mice
treated with combined treatment and Cur-Nano.
Conclusion: Nanoparticles proved effective drug delivery system for Cur against trichinosis with
noticeable outcomes in the muscle phase. The synergistic activity of ABZ/Cur-Nano gives the best results
in both intestinal and muscular phases.
Keywords
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