El Saftawy, E., Ghallab, M., Haydara, T., Ibrahim, A., Atta, S., Gad Allah, R., Kamel, M., Morsy, S. (2023). Assessment of host biochemical factors and microbiota interactions and pathogenicity of Blastocystis hominis genotypes: A cross-sectional study. Parasitologists United Journal, 16(3), 183-191. doi: 10.21608/puj.2023.239538.1224
Enas El Saftawy; Marwa Ghallab; Tamer Haydara; Asmaa Ibrahim; Shimaa Atta; Reham Gad Allah; Marwa Kamel; Salwa Morsy. "Assessment of host biochemical factors and microbiota interactions and pathogenicity of Blastocystis hominis genotypes: A cross-sectional study". Parasitologists United Journal, 16, 3, 2023, 183-191. doi: 10.21608/puj.2023.239538.1224
El Saftawy, E., Ghallab, M., Haydara, T., Ibrahim, A., Atta, S., Gad Allah, R., Kamel, M., Morsy, S. (2023). 'Assessment of host biochemical factors and microbiota interactions and pathogenicity of Blastocystis hominis genotypes: A cross-sectional study', Parasitologists United Journal, 16(3), pp. 183-191. doi: 10.21608/puj.2023.239538.1224
El Saftawy, E., Ghallab, M., Haydara, T., Ibrahim, A., Atta, S., Gad Allah, R., Kamel, M., Morsy, S. Assessment of host biochemical factors and microbiota interactions and pathogenicity of Blastocystis hominis genotypes: A cross-sectional study. Parasitologists United Journal, 2023; 16(3): 183-191. doi: 10.21608/puj.2023.239538.1224
Assessment of host biochemical factors and microbiota interactions and pathogenicity of Blastocystis hominis genotypes: A cross-sectional study
4Biotechnology Research Institute, Sadat City University5,Egypt
5Theodor Bilharz Research Institute,Egypt
6Master in Quality Management, Arab Academy for Science, Technology, and Maritime Transport, Productivity and Quality Institute, Smart Village
7Departments of Medical Parasitology, Faculty of Medicine , Cairo University& Modern University for Technology and Information , Egypt
Abstract
Background: Although several clinical and scientific evidences confirmed B. hominis pathogenicity in irritable bowel syndrome (IBS), host biochemical risk factors involved in IBS manifestations are still ambiguous. Objective: To investigate the interactions between B. hominis genotypes, host biochemical factors, and microbiota. Patients and Methods: In a cross-sectional study, 167 stool samples from patients attending the Internal Medicine Department, of Kafr El-Sheikh University Hospital were examined. Using specific sequencedtagged site (STS) primers, samples positive for B. hominis were subtyped. Reducing sugar and pH were assessed in B. hominis-positive samples. The lactate dehydrogenase (LDH) enzyme was measured in both B. hominis sole infections and blastocystosis coexisting with G. intestinalis. To determine the predominant co-existing organisms in blastocystosis infections, bacterial and fungal stool cultures were performed. Furthermore, laboratory-cultivated B. hominis was incubated in vitro with Escherichia (Esch.) coli and Candida non-albicans to assess possible interactions with models of normal microbiota. Results: Of 167 cases, thirty-three (19.7%) were microscopically positive, and twenty seven (16.2%) were molecularly confirmed harboring B. hominis. Genotype 3 was solely detected with higher prevalence in summer and spring. There was a consistent chemical association of carbohydrate intolerance and acidic pH with genotype-3 B. hominis that seemed to augment IBS-like manifestations. Predominant overgrowth of Esch. coli in cultured stool samples was observed. Co-culture of B. hominis with Esch. coli and C. nonalbicans augmented their growth whereas the parasite was suppressed. Coinfection of B. hominis with G. intestinalis showed a significant rise in LDH enzyme in stool samples compared with the presence of B. hominis alone. Conclusion: Genotype-3 B. hominis is predominant and closely related to IBS-like manifestations. Blastocystosis appeared to be related to carbohydrate intolerance, fecal acidity, and enhanced effect on the growth of Esch. coli and C. non-albicans.
View on SCiNiTO
Top