Abdelmaksoud, H., Nahas, E., Ismail, M., Abu-Sarea, E., El-Askary, H., Zalat, R., Elgohary, S., Ibrahim, S. (2023). Evaluation of the therapeutic effect of Zingiber Officinale loaded on nanoparticles for cryptosporidiosis in experimentally immunosuppressed mice. Parasitologists United Journal, 16(2), 133-138. doi: 10.21608/PUJ.2023.209631.1211
Hagar Abdelmaksoud; Engy Nahas; Mousa Ismail; Enas Abu-Sarea; Hala El-Askary; Rabab Zalat; Shaimaa Elgohary; Shimaa Ibrahim. "Evaluation of the therapeutic effect of Zingiber Officinale loaded on nanoparticles for cryptosporidiosis in experimentally immunosuppressed mice". Parasitologists United Journal, 16, 2, 2023, 133-138. doi: 10.21608/PUJ.2023.209631.1211
Abdelmaksoud, H., Nahas, E., Ismail, M., Abu-Sarea, E., El-Askary, H., Zalat, R., Elgohary, S., Ibrahim, S. (2023). 'Evaluation of the therapeutic effect of Zingiber Officinale loaded on nanoparticles for cryptosporidiosis in experimentally immunosuppressed mice', Parasitologists United Journal, 16(2), pp. 133-138. doi: 10.21608/PUJ.2023.209631.1211
Abdelmaksoud, H., Nahas, E., Ismail, M., Abu-Sarea, E., El-Askary, H., Zalat, R., Elgohary, S., Ibrahim, S. Evaluation of the therapeutic effect of Zingiber Officinale loaded on nanoparticles for cryptosporidiosis in experimentally immunosuppressed mice. Parasitologists United Journal, 2023; 16(2): 133-138. doi: 10.21608/PUJ.2023.209631.1211
Evaluation of the therapeutic effect of Zingiber Officinale loaded on nanoparticles for cryptosporidiosis in experimentally immunosuppressed mice
2Departments of Parasitology Faculties of Medicine,Beni Suef, Beni Suef, Egypt
3Departments of Parasitology, Faculties of Medicine,Cairo Cairo Egypt
4Departments of Parasitology, Faculties of Medicine. Beni Suef Universities, Beni Suef ,
5Departments of Parasitology, Faculties of Medicine, Beni Suef, Universities, Beni Suef
6Departments of Parasitology, Theodor Bilharz Research Institute , Cairo
7Departments of Parasitology Pathology Faculties of Medicine, Ain Shams , Cairo, Egypt
8Departments of Parasitology, Faculties of Medicine, Beni Suef Universities, Beni Suef
Abstract
Background: Cryptosporidiosis causes retractable diarrhea with drastic dehydration, especially in immunocompromised patients. Search for a novel therapeutic approach is urgently required. Objective: To assess the potential effect of Zingiber Officinale (ginger) loaded on chitosan nanoparticles (ginger CSNPs) versus Nanazoxid® (NTZ) in treatment of cryptosporidiosis. Material and Methods: Seventy mice were immunosuppressed and divided into seven groups (10 mice each), of which two groups were negative and positive control groups. Except for the negative control group, cryptosporidiosis was experimentally induced, and different drug regimens (NTZ, ginger, CSNPs, ginger CSNPs, and NTZ combined with ginger CSNPs) were given for seven consecutive days. Stool samples were collected daily for oocysts count, followed by sacrifice of mice one week after drug administration. Histopathological examination was conducted for small intestine, liver, and lung tissues. Results: Treatment with ginger CSNPs recorded the highest significant reduction in oocysts shedding, followed by treatment with NTZ/ginger CSNPs combined therapy. Histopathological examination of small intestine showed the best amelioration results in mice treated with ginger CSNPs signified by remarkable improvement in the form of intact intestinal mucosa, with complete healing of intestinal surface epithelium, and with preserved brush border and absence of mucosal ulceration. Histopathologic effect in liver showed various degrees of expansion of portal tracts with moderate inflammation among the treated groups. The lung showed congestion with various degrees of emphysematous changes and inflammatory infiltration among the treated groups. Conclusion: Ginger CSNPs possessed anti-cryptosporidial activity, diminished the oocysts shedding, and protected the intestinal epithelial from deleterious effects of cryptosporidiosis.
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