El-Gebaly, N., Rehan, M., Abdelfattah, D. (2019). Immunomodulating effect of Schistosoma mansoni soluble egg antigen on course of induced diabetes mellitus in experimental mice. Parasitologists United Journal, 12(1), 45-52. doi: 10.21608/puj.10929.1036
Naglaa SM El-Gebaly; Mohamed K Rehan; Dina S Abdelfattah. "Immunomodulating effect of Schistosoma mansoni soluble egg antigen on course of induced diabetes mellitus in experimental mice". Parasitologists United Journal, 12, 1, 2019, 45-52. doi: 10.21608/puj.10929.1036
El-Gebaly, N., Rehan, M., Abdelfattah, D. (2019). 'Immunomodulating effect of Schistosoma mansoni soluble egg antigen on course of induced diabetes mellitus in experimental mice', Parasitologists United Journal, 12(1), pp. 45-52. doi: 10.21608/puj.10929.1036
El-Gebaly, N., Rehan, M., Abdelfattah, D. Immunomodulating effect of Schistosoma mansoni soluble egg antigen on course of induced diabetes mellitus in experimental mice. Parasitologists United Journal, 2019; 12(1): 45-52. doi: 10.21608/puj.10929.1036
Immunomodulating effect of Schistosoma mansoni soluble egg antigen on course of induced diabetes mellitus in experimental mice
1Department of Medical Parasitology, Faculty of Medicine, Cairo University, Egypt.
2Department of Internal Medicine, Beni Suef University, Egypt.
3Department of Biochemistry and Biotechnology, Faculty of Medicine, Cairo University, Egypt.
Abstract
Background: Helminth infections, particularly S.mansoni, are known to induce a protective role against various forms of autoimmune diseases, including type 1diabetes (TID). The observed S.mansoni significant inhibition or delay of diabetes development in non-obese diabetic mice (NOD), appeared to be due to a modulation of the diabetes-associated th1response towards protective th2 responses through IL-10 production. Objective: To study the effect of S. mansoni SEA on the immune response in induced TIDmouse moduel. Material and Methods: In this study, 90 male Swiss Albino mice of 6 weeks old, weighing between 90 and 100g were divided into 5 groups; control group (I): Streptozontocin (STZ)-treated group (II); soluble egg antigen (SEA)-immunized group (III); (STZ+SEA) group (IV); (SEA+STZ) group (V). Mice were subjected to measurement of blood glucose levels at two and four weeks by colorimetric method, and measurement of IL-10 by enzyme linked immunosorbent assay (ELISA). Histopathological examination of pancreatic sections of the five groups investigated signs suggesting presence or absence of pancreatic inflammation.
Results: Significant lowering of blood glucose level occurred at 2-weeks in groups III and V compared to group II and at 4-weeks in groups III, IV and V compared to group II, and in group V compared to group IV. Significant higher IL-10 level occurred at 2-weeks in groups IV and V compared to group II, and in groups IV and V compared to group III and in group V compared to group IV. In 4-weeks, significant increase in IL-10 level occurred in groups II, IV, V compared to group I, and in group V compared to group IV. No significant difference between groups III and I was recorded. Histopathological changes of pancreatic sections of groups I and III showed normal architecture of pancreatic cells; While groups II and IV coinciding with STZ treatment showed vacuolation and necrosis of islets of Langerhans at 2-weeks the inflammation subsided in group IV. In group V there was dilation of blood vessels with inflammatory cells at both weeks. Conclusion: S.mansoni derived SEA proved to be protective against TID leading to improvement of blood sugar control and indicating the protective role of S.mansoni infection.
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