Yahia, S., Moawad, H., Farag, T., Mohammad, S. (2019). Molecular monitoring of the therapeutic effect of Albendazole on Ascaris lumbricoides and Ancylostoma duodenale infected children using conventional multiplex PCR. Parasitologists United Journal, 12(1), 53-60. doi: 10.21608/puj.2019.10309.1033
Samah H Yahia; Howayda SF Moawad; Tahani I Farag; Samira M Mohammad. "Molecular monitoring of the therapeutic effect of Albendazole on Ascaris lumbricoides and Ancylostoma duodenale infected children using conventional multiplex PCR". Parasitologists United Journal, 12, 1, 2019, 53-60. doi: 10.21608/puj.2019.10309.1033
Yahia, S., Moawad, H., Farag, T., Mohammad, S. (2019). 'Molecular monitoring of the therapeutic effect of Albendazole on Ascaris lumbricoides and Ancylostoma duodenale infected children using conventional multiplex PCR', Parasitologists United Journal, 12(1), pp. 53-60. doi: 10.21608/puj.2019.10309.1033
Yahia, S., Moawad, H., Farag, T., Mohammad, S. Molecular monitoring of the therapeutic effect of Albendazole on Ascaris lumbricoides and Ancylostoma duodenale infected children using conventional multiplex PCR. Parasitologists United Journal, 2019; 12(1): 53-60. doi: 10.21608/puj.2019.10309.1033
Molecular monitoring of the therapeutic effect of Albendazole on Ascaris lumbricoides and Ancylostoma duodenale infected children using conventional multiplex PCR
Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Egypt.
Abstract
ABSTRACT Background: Globally, many individuals are infected with soil-transmitted helminthes (STHS)with majority occurring in tropical and subtropical regions of the world. Albendazole (ADZ) is a broad-spectrum anthelmintic used efficiently for STHs control. Upgrading the assessment of ADZ efficacy is important to confirm that drug resistance has not emerged. Objective: To assess the therapeutic efficiency of ADZ in both A.duodenale infections by conventional multiplex PCR. Subjects and Methods: Stool specimens from 191 boys and 123 girls, aged from 2to3 years were screened by saline wet mount and iodine stained smears and cellophane thick smears (Keto-Katz technique). All positive cases were given a single oral dose of 400 mg ADZ than 2 weeks later new stool samples were collected and subjected to multiplex PCR. Before medication, anemia assessment (figure prick capillary blood sample) was performed. Results: Prevalence of infection with A.lumbricoides and A.duodenale was 20.7% and 5.4%, respectively. Parasitic infections were more prevalent in age group 7-13 years (59%) than in 2-6 years (41%) respectively. Boys were slightly more affected than girls (52.6% versus 47.4%). Prevalence of parasitic infections among cases living in rural areas proved to higher than those living in urban areas (65.4% versus 34.6%). Examination for associated anemia showed that 35.9% of infected cases were anemic (Hb level < 11.5 mg/dl) versus 9.7% in non-infected cases. Diarrhea and colic were more prevalent in infected cases (39.7% and 62.8%, respectively) than in those that were non-infected (5.5% and 8.9%, respectively). The therapeutic efficacy of ADZ resulted in a cure rate of 93.8% and 88.2% for A.lumbricoides and A.duodenal infections, respectively; with an egg reduction rate (ERR)of 96.1% and 91.2%, respectively. The multiplex PCR sensitivity was 100% for detection of both worms; the specificity was 96.8% and 93.8%, respectively. With diagnostic accuracy of 96.9% and 94.1%, respectively. Conclusion: Multiplex PCR was useful for measurement of the therapeutic efficacy of ADZas an anthelminthic drug.
View on SCiNiTO
Top