Abdeltawab, M., Abdel-Shafi, I., Aboulhoda, B., Wanas, H., Saad El-Din, S., Amer, S., Hamed, A. (2022). Investigating the effect of the nitric oxide donor L-arginine on albendazole efficacy in Trichinella spiralis-induced myositis and myocarditis in mice. Parasitologists United Journal, 15(1), 60-70. doi: 10.21608/puj.2022.119638.1153
Magda Abdeltawab; Iman Abdel-Shafi; Basma Aboulhoda; Hanaa Wanas; Shimaa Saad El-Din; Samar Amer; Alshaimaa Hamed. "Investigating the effect of the nitric oxide donor L-arginine on albendazole efficacy in Trichinella spiralis-induced myositis and myocarditis in mice". Parasitologists United Journal, 15, 1, 2022, 60-70. doi: 10.21608/puj.2022.119638.1153
Abdeltawab, M., Abdel-Shafi, I., Aboulhoda, B., Wanas, H., Saad El-Din, S., Amer, S., Hamed, A. (2022). 'Investigating the effect of the nitric oxide donor L-arginine on albendazole efficacy in Trichinella spiralis-induced myositis and myocarditis in mice', Parasitologists United Journal, 15(1), pp. 60-70. doi: 10.21608/puj.2022.119638.1153
Abdeltawab, M., Abdel-Shafi, I., Aboulhoda, B., Wanas, H., Saad El-Din, S., Amer, S., Hamed, A. Investigating the effect of the nitric oxide donor L-arginine on albendazole efficacy in Trichinella spiralis-induced myositis and myocarditis in mice. Parasitologists United Journal, 2022; 15(1): 60-70. doi: 10.21608/puj.2022.119638.1153
Investigating the effect of the nitric oxide donor L-arginine on albendazole efficacy in Trichinella spiralis-induced myositis and myocarditis in mice
1Departments of Medical Parasitology,Faculty of Medicine, Cairo University, Cairo 11956, Egypt
2Departments of Anatomy and Embryology , Faculty of Medicine, Cairo University, Cairo 11956, Egypt
3Departments of Pharmacology , Faculty of Medicine, Cairo University, Cairo 11956, Egypt
4Departments of Biochemistry4, Faculty of Medicine, Cairo University, Cairo 11956, Egypt
5Departments of Pathology , Faculty of Medicine, Cairo University, Cairo 11956, Egypt
6Departments of Medical Parasitology , Faculty of Medicine, Cairo University, Cairo 11956, Egypt
Abstract
Background: The immune response against Trichinella spiralis results in a mixed cytokine state and nitric oxide (NO) production. Albendazole (ALB) influences the oxidative status and cytokine profile of the host, in addition to its direct action on the parasite β-tubulin. Objective: The current study was designed to assess the effect of the NO donor, L-arginine, on the muscular phase of experimental trichinosis in mice with and without ALB administration. Material and Methods: The study included five groups (G); negative non-infected control group (G1); positive infected non-treated control group (G2); ALB-treated group (G3); L-arginine-treated group < br />(G4); and combined ALB and L-arginine regimen group (G5). Study parameters included larval count, histopathological changes, and NO synthase (iNOS) immunohistochemical expression in skeletal muscle and heart during the muscular phase. Gene expression levels of tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), and β-tubulin, as well as NO production were evaluated by quantitative real time PCR (RT-qPCR). Results: L-arginine resulted in a significant increase in tissue iNOS expression and serum NO levels, in addition to the increase in TNF and INF gene expression. Albendazole, on the other hand, was found to decrease local tissue expression of iNOS and serum NO in infected mice, whereas gene expression levels of TNF and IFN were elevated. Conclusion: Nitric oxide homeostasis is vital to the infection outcome and regeneration during muscular trichinosis. Albendazole exerts immunomodulatory effects in addition to its direct anti-parasitic action on T. spiralis.
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