Kishik, S., Nagati, I., El Hayawan, I., Ali, I., Fawzy, M., Ali, H. (2020). Efficacy of Nigella sativa oil and its chitosan loaded nanoparticles on experimental cystic echinoncoccosis with immunological assessment. Parasitologists United Journal, 13(3), 172-178. doi: 10.21608/PUJ.2020.41053.1086
Shereen Kishik; Ibrahim Nagati; Ibrahim El Hayawan; Ibrahim Ali; Maha Fawzy; Hemat Ali. "Efficacy of Nigella sativa oil and its chitosan loaded nanoparticles on experimental cystic echinoncoccosis with immunological assessment". Parasitologists United Journal, 13, 3, 2020, 172-178. doi: 10.21608/PUJ.2020.41053.1086
Kishik, S., Nagati, I., El Hayawan, I., Ali, I., Fawzy, M., Ali, H. (2020). 'Efficacy of Nigella sativa oil and its chitosan loaded nanoparticles on experimental cystic echinoncoccosis with immunological assessment', Parasitologists United Journal, 13(3), pp. 172-178. doi: 10.21608/PUJ.2020.41053.1086
Kishik, S., Nagati, I., El Hayawan, I., Ali, I., Fawzy, M., Ali, H. Efficacy of Nigella sativa oil and its chitosan loaded nanoparticles on experimental cystic echinoncoccosis with immunological assessment. Parasitologists United Journal, 2020; 13(3): 172-178. doi: 10.21608/PUJ.2020.41053.1086
Efficacy of Nigella sativa oil and its chitosan loaded nanoparticles on experimental cystic echinoncoccosis with immunological assessment
1Departments of Parasitology , Faculty of Medicine, Benha University, Benha,
2Immunoparasitology , Theodor Bilharz Research institute, Imbaba, Giza, Egypt
3Departments of Parasitology , Faculty of Medicine, Benha University, Benha
Abstract
Background: Cystic echinococcosis is a frequent worldwide disease. Surgery is the main policy of treatment. The benzimidazoles are the drugs of choice as adjuvant therapy besides surgery, but they have some drawbacks, so there is a pressing need to develop other safe and cheap drugs such as an herbal extract. Objectives: To increase the efficacy of Nigella sativa oil (NSO) administered either as monotherapy or combined with albendazole, and using chitosan nanoparticles as a drug delivery system for both drugs. Material and Methods: E. granulosus protoscoleces were obtained by aseptic puncture of hydatid cysts. Mice infected by intraperitoneal inoculation with nearly 1000 protoscoleces per mouse were subdivided into 7 groups: G2, infected non-treated; G3, treated with albendazole; G4, treated with NSO; G5, treated with NSO and albendazole; G6, treated with albendazole loaded on chitosan nanoparticles; G7, treated with NSO loaded on chitosan nanoparticles; G8, treated with albendazole and NSO loaded on chitosan nanoparticles. G1 included the non-infected, non-treated control mice. Serum was collected for biochemical and serological assay. NO was measured according to nitric oxide assay colorimetric kit. The systemic level of TNF-α and IL-5 was determined in the plasma of mice using commercial enzyme-linked immunosorbent assay kits. Results: The results revealed that a significant increase of IL-5 occurred in all treated groups to reach the highest value in G8. There was a significant decrease of TNF-α in all treated groups in comparison to G1 and G2 (P<0.001). Mean level of nitric oxide (NO) was significantly increased in groups loaded on chitosan nanoparticles in comparison to G2. Conclusion: Nigella sativa oil showed a promising effect on CE when used alone or in combination with albendazole and its effect was augmented when loaded on chitosan nanoparticles.
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