El-Taweel, H., Isaa, Y., Shehata, G., Gaballah, A., Lotfy, W., Tolba, M. (2020). Restriction fragment length polymorphism (RFLP) analysis of Blastocystis spp. in symptomatic and asymptomatic individuals from Alexandria, Egypt. Parasitologists United Journal, 13(3), 164-171. doi: 10.21608/puj.2020.38378.1084
Hend El-Taweel; Yasmine Isaa; Ghada Shehata; Ahmed Gaballah; Wael Lotfy; Mona Tolba. "Restriction fragment length polymorphism (RFLP) analysis of Blastocystis spp. in symptomatic and asymptomatic individuals from Alexandria, Egypt". Parasitologists United Journal, 13, 3, 2020, 164-171. doi: 10.21608/puj.2020.38378.1084
El-Taweel, H., Isaa, Y., Shehata, G., Gaballah, A., Lotfy, W., Tolba, M. (2020). 'Restriction fragment length polymorphism (RFLP) analysis of Blastocystis spp. in symptomatic and asymptomatic individuals from Alexandria, Egypt', Parasitologists United Journal, 13(3), pp. 164-171. doi: 10.21608/puj.2020.38378.1084
El-Taweel, H., Isaa, Y., Shehata, G., Gaballah, A., Lotfy, W., Tolba, M. Restriction fragment length polymorphism (RFLP) analysis of Blastocystis spp. in symptomatic and asymptomatic individuals from Alexandria, Egypt. Parasitologists United Journal, 2020; 13(3): 164-171. doi: 10.21608/puj.2020.38378.1084
Restriction fragment length polymorphism (RFLP) analysis of Blastocystis spp. in symptomatic and asymptomatic individuals from Alexandria, Egypt
1Departments of Parasitology,Medical Research Institute,Alexandria University
2Departments of Biochemistry,Faculties of Medicine,Alexandria University
3Departments of Biochemistry,Faculties of Science, Alexandria University
4Departments of Microbiology, Medical Research Institute,Faculties of Science,Alexandria University
5Departments of Community Health,Faculties of Nursing , Matrouh University , Egypt
6Departments of Parasitology, Medical Research Institute , Alexandria University
Abstract
Background: Blastocystis spp. is a unique enteric parasite commonly found in the intestines of humans and animals. In humans, prevalence up to 60% has been reported in tropical, subtropical, and developing countries. Currently 26 subtypes (STs) of Blastocystis have been described based on sequence analysis, 9 were reported in humans. Objectives: The aim of the work was to determine the different genotypes of Blastocystis spp. in symptomatic and asymptomatic individuals in Alexandria city, Egypt. Subjects and Methods: Examination of 100 stool samples was performed to detect Blastocystis collected from patients complaining of gastrointestinal (GI) disturbances and asymptomatic individuals. PCR restriction fragment length polymorphism analysis (PCR-RFLP) and sequencing of amplified products was performed for Blastocystis subtyping. Results: Out of 47 fecal samples positive only for Blastocystis spp. by microscopy, 39 patients presented with symptoms and 8 were asymptomatic participants. Blastocystis small subunit rRNA (SSU rRNA) was successfully amplified from 27 samples; 24/39 symptomatic and 3/8 asymptomatic. Among symptomatic patients, four STs were identified; ST3 was the most common (55%) followed by ST1 (20%), ST4 (15%) and ST2 (10%). While ST2 and ST4 were identified only in patients having GI symptoms, ST1 and ST3 were found in both symptomatic and asymptomatic participants. Amplicons of 7 samples from symptomatic patients were not digested after incubation with the restriction enzymes and could not be genotyped. Conclusion: Genotyping of Blastocystis spp. from fecal samples revealed the presence of four different subtypes: ST1, ST2, ST3 and ST4 with predominance of ST3. No statistically significant association could be observed between Blastocystis STs and clinical presentation of the studied subjects.
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